Related Experiment Video
Updated: Dec 12, 2025
Mass Spectrometric Analysis of Glycosphingolipid Antigens
Published on: April 16, 2013
Detection of Functionalized Sphingolipid Analogs in Detergent-Resistant Membranes of Immune Cells
Julian Fink1, Jürgen Seibel1, Elita Avota2
1Institute of Organic Chemistry, University of Wuerzburg, Wuerzburg, Germany.
Abstract:
The analysis of protein enrichment in the detergent-resistant membranes (DRMs) isolated from immune cells enables us to analyze a link between the membrane lipid dynamics and cell activation. Here, we describe the fractionation of detergent-resistant membranes and the correlative analysis of the enrichment of T cell receptor (TCR) and ω-azido-modified synthetic ceramide in those fractions upon TCR stimulation.
More Related Videos
08:46Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins
Published on: September 22, 2020
07:33Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction
Published on: April 26, 2011