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Related Concept Videos

Instrument Calibration01:12

Instrument Calibration

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Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...
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Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

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Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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Updated: Dec 12, 2025

Construction and Setup of a Bench-scale Algal Photosynthetic Bioreactor with Temperature, Light, and pH Monitoring for Kinetic Growth Tests
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Process adapted calibration improves fluorometric pH sensor precision in sophisticated fermentation processes.

Joseph Newton1, Reinhard Oeggl1, Nils H Janzen1

  • 1Boehringer Ingelheim RCV GmbH & Co. KG Vienna Austria.

Engineering in Life Sciences
|August 11, 2020
PubMed
Summary
This summary is machine-generated.

Biomass interferes with fluorometric pH sensors used in miniaturized bioreactors. A new calibration method improves pH control accuracy for complex microbial fermentation processes.

Keywords:
automationfluorometric pH sensorhigh‐throughputmicrobial fermentationmicrobioreactorscale‐down model

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Area of Science:

  • Biotechnology
  • Bioprocess Engineering
  • Microbial Fermentation

Background:

  • Miniaturization and automation are key trends in bioprocess development for high-throughput screening.
  • Complex fed-batch fermentation strategies, including pH and temperature shifts, are increasingly used for optimal protein expression.
  • Accurate scale-down models require small-scale bioreactors to replicate complex process designs.

Purpose of the Study:

  • To investigate the impact of biomass on the accuracy of fluorometric pH sensors.
  • To develop and present a calibration methodology for precise online pH measurement in the presence of biomass.
  • To provide recommendations for enhanced pH control in sophisticated fermentation processes.

Main Methods:

  • Evaluation of a commercially available fluorometric pH sensor's performance.
  • Analysis of biomass-induced alterations in sensor calibration curves.
  • Development and validation of a novel calibration methodology for biomass-containing samples.

Main Results:

  • Biomass fluorescence was found to alter the sigmoidal calibration curve of fluorometric pH sensors.
  • This alteration leads to inaccurate pH measurements and control in microbial fermentations.
  • The proposed calibration methodology demonstrated improved accuracy for online pH monitoring.

Conclusions:

  • Biomass interference is a significant challenge for fluorometric pH sensing in bioprocesses.
  • Accurate pH control in miniaturized bioreactors necessitates addressing biomass-related signal distortions.
  • The developed calibration approach offers a viable solution for reliable online pH monitoring in complex fermentation systems.