Suzuki Cross-Coupling Reaction with Genetically Encoded Fluorosulfates for Fluorogenic Protein Labeling

Area of Science:

• Biochemistry
• Chemical Biology
• Molecular Imaging

Background:

• Palladium-catalyzed cross-coupling reactions are established for protein modification.
• Existing methods may lack efficiency or biocompatibility for certain applications.
• Novel fluorogenic approaches are needed for sensitive protein detection.

Purpose of the Study:

• To develop a new fluorogenic protein modification methodology.
• To enable responsive "off/on" fluorescence switching for protein imaging.
• To demonstrate high efficiency and biocompatibility in bacterial cells and aqueous media.

Main Methods:

• Genetically encoding fluorosulfate-L-tyrosine in proteins.
• Utilizing palladium-catalyzed cross-coupling with aryl halide functionalities.
• Performing cross-coupling of 4-cyanophenylboronic acid on green fluorescent protein.

Main Results:

• Achieved efficient and biocompatible protein modification in bacterial cells and aqueous environments.
• Demonstrated a unique fluorogenic property with an "off/on" fluorescence switch.
• Showcased minimal background noise for spectroscopic protein imaging.

Conclusions:

• Developed a convenient and efficient catalytic system for protein visualization.
• The new methodology offers broad utilities for live-cell imaging.
• This approach has significant potential for advanced molecular imaging techniques.