Pseudomonas viciae sp. nov., isolated from rhizosphere of broad bean

A Gram-stain-negative aerobic bacterium, strain 11K1^T, was isolated from a rhizosphere soil of broad bean collected from Qujing, Yunnan, PR China and characterized by using polyphasic taxonomy. The bacterial cells of strain 11K1^T were rod-shaped, motile by two polar flagella and positive for oxidase and catalase. Results of phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain had the highest similarities to Pseudomonas thivervalensis DSM 13194^T (99.52 %), Pseudomonas lini CFBP 5737^T (99.45 %), Pseudomonas chlororaphis subsp. chlororaphis NBRC 3904^T (99.31 %), Pseudomonas kilonensis DSM 13647^T (99.25 %) and Pseudomonas brassicacearum JCM11938^T (99.24 %). Multilocus sequence analysis using the 16S rRNA, gyrB, rpoB and rpoD gene sequences demonstrated that strain 11K1^T was a member of the Pseudomonas corrugata subgroup within the Pseudomonas fluorescens lineage, but was distant from all closely related species. The average nucleotide identity and in silico DNA-DNA hybridization values were lower than recommended thresholds of 95 and 70 %, respectively, for species delineation. The major isoprenoid quinone of strain 11K1^T was ubiquinone (Q-9) and the major cellular fatty acids were C_16 : 0, summed feature 3 (C_16 : 1 ω7c/C_16 : 1 ω6c), summed feature 8 (C_18 : 1 ω7c/C_18 : 1 ω6c) and C_17 : 0 cyclo. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, aminophospholipid and two unidentified lipids. Based on the results of phenotypic characterization, phylogenetic analysis and genome comparison, strain 11K1^T represents a novel species of the genus Pseudomonas, for which the name Pseudomonas viciae sp. nov. is proposed. The type strain is 11K1^T (=GDMCC 1.1743^T=KACC 21650^T).