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Cryoprotective activity of phosphorus-containing phenol

M N Kolyada1, V P Osipova1, N T Berberova2

  • 1Southern Scientific Center, Russian Academy of Sciences, Str. Chehova 41, Rostov-on-Don, 344006, Russia.

Cryobiology
|August 11, 2020
PubMed

Insights

The antioxidant (MDPA) shows varied cryoprotective effects on sturgeon sperm. This compound reduces lipid peroxidation, improving sperm activity and fertility after deep freezing.

Area of Science:

  • Aquatic Biology
  • Biochemistry
  • Reproductive Science

Background:

  • Cryopreservation of fish sperm is crucial for aquaculture and conservation.
  • Sturgeon sperm are particularly sensitive to cryopreservation damage.
  • Lipid peroxidation is a major cause of sperm damage during cryopreservation.

Purpose of the Study:

  • To evaluate the antioxidant and cryoprotective efficiencies of MDPA in sturgeon sperm.
  • To compare the effects of MDPA across different sturgeon species: Russian sturgeon, beluga, and Stellate sturgeon.
  • To assess MDPA's impact on sperm lipid peroxidation, activity, and fertility post-cryopreservation.

Main Methods:

  • Sperm samples from Russian sturgeon, beluga, and Stellate sturgeon were cryopreserved.
  • The cryoprotective agent 3,5-di-tert-butyl-4-hydroxyphenyl)methylenediphosphonic acid (MDPA) was used.
  • Lipid peroxidation levels, sperm motility, viability, and fertility rates were measured before and after freezing.

Main Results:

  • MDPA demonstrated significant antioxidant and cryoprotective effects, but efficiency varied among sturgeon species.
  • MDPA effectively decreased lipid peroxidation in sturgeon sperm.
  • Improved sperm activity and fertility were observed for both native and cryopreserved sturgeon sperm treated with MDPA.

Conclusions:

  • MDPA is a promising cryoprotectant for sturgeon sperm, reducing oxidative stress and improving post-thaw quality.
  • Species-specific responses to MDPA highlight the need for tailored cryopreservation protocols for different sturgeon.
  • Further research into MDPA's mechanism and optimization for various sturgeon species is warranted for effective sperm banking.
Keywords:
AntioxidantArtificial inseminationBelugaCryopreservationPhosphorus-containing phenolRussian sturgeonSperm cellsStellate sturgeon

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