Related Experiment Video
Updated: Aug 9, 2025

Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling
Published on: March 4, 2018
Use of cryoprotectors and antioxidants in sturgeon semen cryopreservation
M N Kolyada1, V P Osipova1, N T Berberova2
1Southern Scientific Centre of Russian Academy of Science, 41 Chekhova str., 344006, Rostov-on-Don, Russia.
Abstract:
In recent years, the possibility of increasing the low cryoresistance of sturgeon sperm by using antioxidants in basic cryoprotective media has been studied. The goal of this work was to review the current literature on impact of the cryoprotectors, well-known antioxidants and new multifunctional compounds on the activity indicators and fertilizing capability of sperm, as well as on biomarkers of cryostress. A special attention is given to the radical scavenging activity of studied compounds in relation to the highly reactive oxygen species, to prevent and negate oxidative stress damage of sturgeon sperm. Also, new trends for future research through the application of novel polyfunctional antioxidants to sturgeon sperm cryopreservation were indicated.

