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Limited proteolysis of actin by a specific bacterial protease
Khaitlina SYu1, T D Smirnova, A M Usmanova
1Institute of Cytology, USSR Academy of Sciences, Leningrad.
FEBS Letters
|February 8, 1988
Abstract:
A 36 kDa fragment of rabbit skeletal muscle actin resistant to further proteolytic breakdown was obtained with a new bacterial protease. This fragment was the only cleavage product obtained from native actin whereas proteolysis of heat-inactivated actin was unlimited. The 36 kDa fragment failed to polymerize and to inhibit DNase I activity. Binding to DNase I protects actin against proteolysis by protease. The results on actin proteolysis by different proteases are compared.