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Updated: Dec 12, 2025

Aip1p Dynamics Are Altered by the R256H Mutation in Actin
Published on: July 30, 2014
Actin chromobody imaging reveals sub-organellar actin dynamics
Cara R Schiavon1,2, Tong Zhang1, Bing Zhao3
1Waitt Advanced Biophotonics Center, Salk Institute for Biological Studies, La Jolla, CA, USA.
Abstract:
The actin cytoskeleton plays multiple critical roles in cells, from cell migration to organelle dynamics. The small and transient actin structures regulating organelle dynamics are challenging to detect with fluorescence microscopy, making it difficult to determine whether actin filaments are directly associated with specific membranes. To address these limitations, we developed fluorescent-protein-tagged actin nanobodies, termed 'actin chromobodies' (ACs), targeted to organelle membranes to enable high-resolution imaging of sub-organellar actin dynamics.
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