A novel electrochemical glucose biosensor based on a poly (L-aspartic acid)-modified carbon-paste electrode

A new amperometric biosensor was fabricated by means of electropolymerization of L-aspartic acid on a carbon-paste electrode (CPE) for the bioelectrochemical determination of glucose. The electropolymerization process was conducted via cyclic voltammetry (CV). The modified CPE with poly (L-aspartic acid) (PAA) provided free carboxyl groups so as to immobilize the glucose oxidase (GOx), and further, enhanced the electrocatalytic activity of the hydrogen peroxide (H₂O₂). The biosensor displayed both good stability and good bioactivity. The sensitivity of the prepared biosensor was 5.3 µA cm^-2mM^-1. Its linear range extended from 0.05 mM to 1.0 mM, with the low limit of detection (LOD) being 69.2 µM. The Michaelis-Menten constant was found to be 1.17 mM. Furthermore, the biosensor showed good anti-interference ability in relation to dopamine, uric acid, and ascorbic acid. Taken together, these results demonstrate that PAA/CPE is a promising material for the fabrication of glucose biosensor.