Mutation-Simulator: fine-grained simulation of random mutations in any genome
M A Kühl1, B Stich1, D C Ries1
1Quantitative Genetics and Genomics of Plants, Heinrich Heine University, Düsseldorf 40225, Germany.
Bioinformatics (Oxford, England)
|August 12, 2020
Summary
Mutation-Simulator introduces sequence alterations in reference genomes efficiently. This tool aids in mimicking natural mutation patterns for high-throughput sequencing applications.
Area of Science:
- Genomics
- Bioinformatics
Background:
- Accurate simulation of genetic variations is crucial for analyzing high-throughput sequencing data.
- Existing tools may face computational challenges with large eukaryotic genomes.
Purpose of the Study:
- To present Mutation-Simulator, a novel tool for introducing sequence alterations.
- To enable the simulation of natural mutation patterns with fine-grained control.
Main Methods:
- Mutation-Simulator utilizes Python 3 for implementation.
- It accepts standard file formats for input and output.
- The software offers control over mutation rates along sequences.
Main Results:
- Mutation-Simulator efficiently handles large eukaryotic genomes.
- It allows for the introduction of diverse sequence alterations.
- The tool facilitates the mimicking of natural mutation patterns.
Conclusions:
- Mutation-Simulator is a valuable, free, and accessible tool for bioinformatics workflows.
- Its design supports integration into development and benchmarking of sequencing applications.
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