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Integrative analysis of microRNA and mRNA expression profiles in MARC-145 cells infected with PRRSV

Zhenpu Liang1, Liang Wang1, Hui Wu1

  • 1College of Life Sciences, Henan Agricultural University, Zhengzhou, 450000, China.

Virus Genes
|August 14, 2020
PubMed

Insights

This study reveals that two specific microRNAs (miRNAs), miR-21-3p and miR-27a-5p, promote Porcine reproductive and respiratory syndrome virus (PRRSV) replication. Understanding these miRNA-host interactions offers new avenues for PRRSV therapeutic development.

Area of Science:

  • Virology
  • Molecular Biology
  • Gene Regulation

Background:

  • Porcine reproductive and respiratory syndrome virus (PRRSV) causes significant global economic losses in the swine industry.
  • MicroRNAs (miRNAs) are key regulators of gene expression with complex interactions during viral infections.
  • A comprehensive analysis of miRNA and mRNA networks in PRRSV-infected cells was lacking.

Purpose of the Study:

  • To investigate and integrate miRNA and mRNA expression profiles and their networks in MARC-145 cells following PRRSV infection.
  • To identify specific miRNAs and their target mRNAs involved in the host response to PRRSV.
  • To elucidate the functional role of identified miRNAs in PRRSV replication and host-virus interactions.

Main Methods:

  • Differential expression analysis of miRNAs and mRNAs in PRRSV-infected MARC-145 cells using RNA-sequencing.
  • Construction and analysis of miRNA-mRNA interaction networks.
  • Gene Ontology (GO) and KEGG pathway enrichment analysis.
  • Validation of RNA-seq results using real-time PCR.
  • Experimental validation of specific miRNA functions in PRRSV replication.

Main Results:

  • Identified 22 differentially expressed miRNAs (DEmiRs) and 390 potential target genes from differentially expressed mRNAs (DEmRs).
  • Constructed miRNA-mRNA interaction networks, predicting 34 KEGG pathways, with 12 linked to viral infection.
  • Validated that miR-27a-5p and miR-21-3p target SPARC, CLIC1, cofilin-1, and COX7A2, and significantly promote PRRSV replication.

Conclusions:

  • This study is the first to report the involvement of miR-21-3p and miR-27a-5p in the host-PRRSV interaction.
  • These miRNAs play a crucial role in promoting PRRSV replication.
  • The findings provide valuable insights into miRNA-mediated regulation during PRRSV infection, potentially aiding in novel therapeutic strategies.
Keywords:
Integrated analysisMARC-145PRRSVmRNAmiRNA

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