Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

362
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
362
Bacterial Signaling01:30

Bacterial Signaling

39.3K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
39.3K
Global Regulatory Systems01:28

Global Regulatory Systems

413
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
413
Autocrine Signaling01:01

Autocrine Signaling

51.2K
Autocrine signaling is one of the many signaling mechanisms that function inside multicellular organisms to carry out intercellular communication. In this type of signaling mechanism, the same cell that secretes an extracellular signaling molecule also expresses the receptors to bind and respond to that signaling molecule.
Autocrine Signaling in Macrophages
Under normal physiological conditions, autocrine signaling is essential for maintaining homeostasis. This process is well characterized in...
51.2K
Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

16.5K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
16.5K
Cascaded Op Amps01:16

Cascaded Op Amps

966
Operational amplifiers (op-amps) are versatile electronic components that can be interconnected in a cascade - one after another in a linear sequence. This cascading is possible due to their infinite input resistance and zero output resistance, allowing them to maintain their input-output relationships even when connected in series.
In a cascaded system, each op-amp is referred to as a stage. The output of one stage drives the input of the subsequent stage. As the input signal passes through...
966

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering 2,4-Diacetylphloroglucinol-Hyper-Tolerant <i>Escherichia coli</i> via Adaptive Evolution for Efficient Biosynthesis and an Organic Solvent-Free Downstream Process.

ACS synthetic biology·2026
Same author

Construction of a pathway-independent three-module self-induced regulatory system and its application in 3-hydroxypropionic acid production.

Engineering microbiology·2026
Same author

FABP4 as an immunometabolic hub in preeclampsia: from maternal-fetal interface to systemic inflammation.

Frontiers in immunology·2026
Same author

Co-infection with hepatitis B and human immunodeficiency virus: epidemiology, pathogenesis, and treatment.

Infectious diseases & immunity·2026
Same author

Synergistic dual ring-cleavage pathways enable efficient degradation of chlorobenzenes by Pseudomonas putida BS-1 in groundwater.

Journal of hazardous materials·2026
Same author

Peroxisomal Surface Display of Cat2 in <i>Yarrowia lipolytica</i> Enhances the Biosynthesis Acetyl-CoA-Derived Chemicals.

Journal of agricultural and food chemistry·2026

Related Experiment Video

Updated: Dec 12, 2025

Large Scale Energy Efficient Sensor Network Routing Using a Quantum Processor Unit
05:30

Large Scale Energy Efficient Sensor Network Routing Using a Quantum Processor Unit

Published on: September 8, 2023

973

Two Completely Orthogonal Quorum Sensing Systems with Self-Produced Autoinducers Enable Automatic Delayed Cascade

Wei Jiang1, Xinyuan He1, Yue Luo1

  • 1State Key Laboratory of Microbial Technology, Shandong University, 266237 Qingdao, China.

ACS Synthetic Biology
|August 14, 2020
PubMed
Summary

Researchers developed two orthogonal quorum sensing (QS) systems for complex environments. This synthetic biology advance enables precise, sequential gene expression without external inducers, advancing biotechnological applications.

Keywords:
automatic delayed cascade controlpromoter orthogonalityquorum sensing systemsignal orthogonality

More Related Videos

Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response
06:26

Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response

Published on: May 23, 2020

8.7K
Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

9.6K

Related Experiment Videos

Last Updated: Dec 12, 2025

Large Scale Energy Efficient Sensor Network Routing Using a Quantum Processor Unit
05:30

Large Scale Energy Efficient Sensor Network Routing Using a Quantum Processor Unit

Published on: September 8, 2023

973
Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response
06:26

Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response

Published on: May 23, 2020

8.7K
Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

9.6K

Area of Science:

  • Synthetic Biology
  • Microbial Systems Engineering
  • Genetic Circuit Design

Background:

  • Quorum sensing (QS) systems regulate bacterial behavior but suffer from signal crosstalk, limiting their use in complex environments.
  • Achieving signal and promoter orthogonality is crucial for predictable control of multiple QS systems within a single cell.
  • Existing QS systems often require exogenous inducers, complicating applications demanding autonomous control.

Purpose of the Study:

  • To engineer two completely orthogonal quorum sensing systems within a single cell.
  • To achieve signal and promoter orthogonality between the engineered QS systems.
  • To demonstrate the utility of these orthogonal QS systems in an intracellular automatic delayed cascade circuit.

Main Methods:

  • Designed two QS systems based on the las system (Pseudomonas aeruginosa) and the tra system (Agrobacterium tumefaciens).
  • Employed synthetic biology strategies and high-throughput screening to optimize signal and promoter orthogonality.
  • Integrated the orthogonal QS systems intracellularly to construct a delayed gene expression cascade circuit.

Main Results:

  • Successfully established two fully orthogonal QS systems within a single bacterial cell.
  • Demonstrated an automatic delayed cascade circuit enabling sequential gene expression without exogenous inducers.
  • Validated the orthogonality of signals and promoters through rigorous screening and application.

Conclusions:

  • Engineered orthogonal QS systems overcome crosstalk limitations, expanding their application scope in complex environments.
  • The developed automatic delayed cascade circuit offers a novel tool for precise, sequential gene control in synthetic biology.
  • This work highlights the potential of orthogonal QS systems for advanced biotechnological applications, including metabolic engineering and dynamic gene regulation.