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A low-interference, high-resolution multianalyte electrochemical biosensor.

Sara L Melow1, Dusty R Miller, Evan A Gizzie

  • 1Department of Chemistry, Vanderbilt University, Nashville, TN 37235-1822, USA. d.cliffel@vanderbilt.edu.

Analytical Methods : Advancing Methods and Applications
|August 14, 2020
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Summary
This summary is machine-generated.

This study developed an advanced electrochemical biosensor using an osmium-based redox polymer. The new sensor effectively detects glucose and lactate with high selectivity and sensitivity, even in the presence of acetaminophen interference.

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Area of Science:

  • Electrochemistry
  • Biosensors
  • Biomedical Engineering

Background:

  • Electrochemical sensors are crucial in healthcare but are often hindered by interfering compounds like acetaminophen (APAP).
  • Existing sensors struggle with selectivity and sensitivity issues when APAP is present.
  • Developing sensors with improved performance in complex biological environments is essential.

Purpose of the Study:

  • To create a novel electrochemical biosensor with enhanced selectivity and sensitivity for glucose and lactate detection.
  • To minimize interference from acetaminophen (APAP) in electrochemical measurements.
  • To demonstrate the sensor's utility in monitoring cellular metabolism under biologically relevant conditions.

Main Methods:

  • Utilized an osmium-based redox polymer for electrochemical detection.
  • Operated the sensor at a decreased voltage to reduce interference.
  • Tested sensor performance with glucose and lactate analytes in the presence of APAP.
  • Monitored glucose metabolism in AML12 cells treated with APAP and/or insulin.

Main Results:

  • Achieved 40-fold and 200-fold greater selectivity for glucose and lactate, respectively, over APAP.
  • Demonstrated high sensitivity for glucose (0.350 ± 0.006 μA mM⁻¹) and lactate (2.00 ± 0.05 μA mM⁻¹).
  • Maintained operational stability over 7 days with minimal performance decrease.
  • Successfully monitored glucose metabolism in AML12 cells under various treatment conditions.

Conclusions:

  • The developed osmium-based redox polymer sensor offers superior selectivity and sensitivity for glucose and lactate detection.
  • This high-resolution electrochemical biosensor effectively mitigates acetaminophen interference.
  • The platform is suitable for microphysiological monitoring of metabolites in complex biological samples.