Phytohormone responses in pepper (Capsicum annuum L.) leaves under a high density of aphid infestation

The time course response of selected phytohormones has been evaluated in sweet pepper plants (Capsicum annuum L.) submitted to a high density (200 aphids/plant) of aphid (Myzus persicae Sulzer) infestation. Abscisic acid (ABA), salicylic acid (SA), indole-3-acetic acid (IAA), and jasmonates (JAs), including jasmonic acid (JA), jasmonoyl-l-isoleucine (JA-Ile), and cis-OPDA have been simultaneously identified and quantitated by UHPLC-MS/MS in pepper leaf tissue harvested at 3, 8 hours post-infestation (hpi), 1, 2, 4 and 7 days post-infestation (dpi). Infested plants showed a reduction in stem length at 7 dpi and in the number of leaves and leaf width from 4 dpi onwards. JA and JA-Ile significantly increased very early (from 3 hpi) while SA only accumulated at 7 dpi. Despite the high density of infestation, the aphid-induced accumulation of JAs was much lower than the burst typically induced by chewing herbivores. On the other side, ABA peaked in aphid-infested plants at 2 and 4 dpi, while IAA content did not change significantly at any time point. Growth inhibition may be partially explained by the high levels of JAs found in aphid-infested plants. The possibility that the obtained results support the hypothesis of the aphid manipulation of plant metabolism is discussed.