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Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

895
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Photoluminescence: Fluorescence and Phosphorescence01:23

Photoluminescence: Fluorescence and Phosphorescence

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Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
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Using Luciferase to Image Bacterial Infections in Mice
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Seeing (and Using) the Light: Recent Developments in Bioluminescence Technology.

Anna C Love1, Jennifer A Prescher2

  • 1Department of Chemistry, University of California, Irvine, Irvine, CA 92697, USA.

Cell Chemical Biology
|August 16, 2020
PubMed
Summary
This summary is machine-generated.

Bioluminescence imaging uses light-producing enzymes and molecules for sensitive, noninvasive biological visualization. New tools now merge bioluminescence with optogenetics for advanced control and sensing in vivo.

Keywords:
bioluminescenceimagingluciferaseluciferinoptogenetics

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Area of Science:

  • Biotechnology
  • Molecular Imaging
  • Optogenetics

Background:

  • Bioluminescence imaging (BLI) enables sensitive, noninvasive visualization of biological processes in vivo.
  • Traditional BLI applications include cell tracking and gene expression analysis.
  • Recent advances expand BLI capabilities beyond traditional uses.

Purpose of the Study:

  • To review recent advancements in bioluminescence technology.
  • To highlight the development of new luciferins and engineered luciferases.
  • To showcase the integration of bioluminescence with optogenetics.

Main Methods:

  • Review of current literature on bioluminescence and optogenetics.
  • Analysis of novel luciferin substrates and engineered luciferase enzymes.
  • Discussion of applications merging bioluminescence with optogenetics.

Main Results:

  • New luciferins and engineered luciferases expand the scope of optical imaging.
  • Bioluminescent systems are increasingly used for controlling biological processes.
  • Integration with optogenetics enables light-driven cellular signaling.

Conclusions:

  • Bioluminescence technology is rapidly evolving with new tool development.
  • The synergy between bioluminescence and optogenetics opens new avenues for biological research.
  • Bioluminescence is transitioning from a sensing tool to a control mechanism in biological systems.