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Updated: Oct 3, 2026

In vivo Dual Substrate Bioluminescent Imaging
Published on: October 11, 2011
An Open, Integrated Platform for Multiplexed Bioluminescence Microscopy
Lorenzo Scipioni1,2, Belen Torrado1,3, Giulia Tedeschi1,2
1Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California 92617, United States.
Abstract:
We report an imaging package to democratize all-in-one bioluminescence and fluorescence microscopy. The platform comprises three tools: PhasorViewer, a visualization suite to design experiments and identify optimal probe combinations; PhasorScope, an open-source, cost-effective microscopy framework to upgrade conventional microscopes; and PhasorAnalysis, a dedicated, user-friendly analysis pipeline to quantify phasor imaging data. We demonstrate the utility of the platform for multiplexed, simultaneous fluorescence and bioluminescence imaging with readily accessible optical reporters.

