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An improved conjugation method for Pseudomonas syringae

Helen C Neale1, Michelle T Hulin2, Richard J Harrison3

  • 1Centre for Research in Bioscience, Faculty of Health and Applied Sciences, The University of the West of England, Frenchay Campus, Bristol BS16 1QY, UK.

Journal of Microbiological Methods
|August 16, 2020
PubMed

Keywords:
ConjugationPseudomonasTnSeq

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Bacterial conjugation is a mechanism of horizontal gene transfer that enables the exchange of genetic material between bacterial cells through direct contact. This process is facilitated by a donor cell carrying a conjugative plasmid, which encodes genes necessary for pilus formation, DNA replication, and transfer. The conjugative plasmid plays a central role in initiating and executing the transfer of genetic material.The tra region of the conjugative plasmid encodes proteins responsible for...
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