Electroactivity of the Gram-positive bacterium Paenibacillus dendritiformis MA-72

Whilst most of the microorganisms recognized as exoelectrogens are Gram-negative bacteria, the electrogenicity of Gram-positive bacteria has not been sufficiently explored. In this study, the putative electroactivity of the Gram-positive Paenibacillus dendritiformis MA-72 strain, isolated from the anodic biofilm of long-term operated Sediment Microbial Fuel Cell (SMFC), has been investigated. SEM observations show that under polarization conditions P. dendritiformis forms a dense biofilm on carbon felt electrodes. A current density, reaching 5 mA m^-2, has been obtained at a prolonged applied potential of -0.195 V (vs. SHE), which represents 35% of the value achieved with the SMFC. The voltammetric studies confirm that the observed Faradaic current is associated with the electrochemical activity of the bacterial biofilm and not with a soluble redox mediator. The results suggest that a direct electron transfer takes place through the conductive extracellular polymer matrix via pili/nanowires and multiple cytochromes. All these findings demonstrate for the first time that the Gram-positive Paenibacillus dendritiformis MA-72 is a new exoelectrogenic bacterial strain.