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Infectious positive- and negative-strand transcript RNAs from bacteriophage Q beta cDNA clones
P N Shaklee1, J J Miglietta, A C Palmenberg
1Biochemistry Department, University of Wisconsin, Madison 53706.
Virology
|March 1, 1988
Abstract:
Plasmids containing full-length cDNA copies of the Q beta RNA phage genome and flanking T7 promoters were constructed. Positive-strand Q beta RNA, generated by in vitro transcription of these plasmids with T7 RNA polymerase, was infectious to Escherichia coli spheroplasts. The Q beta replicase gene from the cloned DNA was subcloned and expressed in E. coli cells by means of a thermoinducible plasmid. Full-length, negative-strand Q beta transcripts were infectious when transfected into spheroplasts containing the induced replicase gene.