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Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation
Peter Kilbride1, Julie Meneghel2
1Cytiva, Danaher Corporation, Cambridge, UK. peter.kilbride@cytiva.com.
Abstract:
From early dry-ice-based freezers and passive coolers, cryopreservation devices have come a long way. With increasing interest in the field of cryobiology from new scientific applications, the importance of reliable, traceable, and reproducible cold chain devices is sure to increase, ensuring more precise cryopreservation and enabling better post-thaw outcomes, both for the user and for biological samples. As with any cryopreservation process, it is important to optimize each part of the cold chain for each lab's biological samples, cryocontainers used, and logistical restraints. In this chapter we describe how freezing technology can be used for cryopreservation of cells.
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