Related Experiment Video
Updated: Dec 11, 2025
A Simple Fluorescence-based Reporter Assay to Identify Cellular Components Required for Ricin Toxin A Chain RTA Trafficking in Yeast
Published on: December 15, 2017
Persistent luminescence nanorod based luminescence resonance energy transfer aptasensor for autofluorescence-free
Yuan-Yuan Jiang1, Xu Zhao1, Li-Jian Chen1
1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, 214122, China; Institute of Analytical Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Abstract:
Serious ochratoxin A (OTA) contamination necessitates the development of rapid, sensitive and selective analytical methods for its determination in food safety. Herein, we report a persistent luminescence resonance energy transfer (LRET) based aptasensor for the autofluorescence-free detection of OTA. OTA aptamer functionalized persistent luminescence nanorod (PLNR) Zn2GeO4:Mn2+ and the aptamer complementary DNA modified gold nanoparticle (AuNP) were used as the donor and the acceptor, respectively. The developed LRET aptasensor integrated the advantages of the long-lasting persistent luminescence of PLNR, the high selectivity of aptamer and the low probe background of LRET sensors, allowing autofluorescence-free detection of OTA in biological samples with high sensitivity and selectivity. The developed LRET aptasensor gave an excellent linearity in the range of 0.01-10 ng mL-1, the detection limit of 3 pg mL-1 and the precision of 2.7% (RSD, n = 11) at 1 ng mL-1 level. The applicability of the developed aptasensor was demonstrated by analyzing beer samples for OTA with the recoveries of 92.3%-104%.
