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Updated: Dec 11, 2025

Light-driven Enzymatic Decarboxylation
Published on: May 22, 2016
Singlet-Oxygen Generation by Peroxidases and Peroxygenases for Chemoenzymatic Synthesis
Kim N Ingenbosch1,2,3, Stephan Quint4, Melanie Dyllick-Brenzinger4
1Niederrhein University of Applied Sciences, Department of Chemistry and Institute for Coatings and Surface Chemistry, Adlerstrasse 32, 47798, Krefeld, Germany.
Abstract:
Singlet oxygen is a reactive oxygen species undesired in living cells but a rare and valuable reagent in chemical synthesis. We present a fluorescence spectroscopic analysis of the singlet-oxygen formation activity of commercial peroxidases and novel peroxygenases. Singlet-oxygen sensor green (SOSG) is used as fluorogenic singlet oxygen trap. Establishing a kinetic model for the reaction cascade to the fluorescent SOSG endoperoxide permits a kinetic analysis of enzymatic singlet-oxygen formation. All peroxidases and peroxygenases show singlet-oxygen formation. No singlet oxygen activity could be found for any catalase under investigation. Substrate inhibition is observed for all reactive enzymes. The commercial dye-decolorizing peroxidase industrially used for dairy bleaching shows the highest singlet-oxygen activity and the lowest inhibition. This enzyme was immobilized on a textile carrier and successfully applied for a chemical synthesis. Here, ascaridole was synthesized via enzymatically produced singlet oxygen.
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