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Organ specific properties for human urinary alkaline phosphatases.
M Miura1, I Koyama, H Matsuzaki
1Central Clinical Laboratories, Tokyo Metropolitan Police Hospital, Japan.
Clinica Chimica Acta; International Journal of Clinical Chemistry
|January 15, 1988
Summary
Urinary alkaline phosphatases (ALPs) vary by disease, with specific types linked to liver, bone, kidney, or placental origins. Analyzing urinary ALP offers insights into organ health and disease states.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Immunology
Background:
- Alkaline phosphatases (ALPs) are enzymes found in various human tissues.
- Urinary ALP levels and characteristics can indicate physiological or pathological conditions.
Purpose of the Study:
- To re-evaluate the isolation and characteristics of human urinary alkaline phosphatases (ALPs).
- To identify the origins and variations of urinary ALPs in different health and disease states.
Main Methods:
- Physicochemical properties analysis
- Immunological identification
- Electrophoretic separation of ALP isoenzymes
Main Results:
- Urinary ALPs in healthy subjects and hepatoma patients resemble liver/bone ALP.
- Nephritis patients show kidney-like ALP with bone and intestinal ALP.
- Pregnant women exhibit liver, bone, and placental-like ALPs.
- Psychiatric patients on chlorpromazine primarily show bone-like ALP.
- Urinary ALP molecular sizes are generally similar to original ALPs, except in renal failure.
- Urinary ALP activity correlates with serum ALP activity (1:40 ratio).
Conclusions:
- Urinary ALP profiles differ significantly across various physiological and pathological conditions.
- Urinary ALP appears to be a modified form of serum ALP.
- Identifying urinary ALP isoenzymes serves as a valuable biomarker for detecting organ-specific disturbances and diseases.