Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Direct and indirect electron transfer between electrodes and redox proteins.

J E Frew1, H A Hill

  • 1Genetics International (UK) Inc., Abingdon.

European Journal of Biochemistry
|March 1, 1988
PubMed
Summary

Direct electrochemistry of redox proteins is achievable with careful electrode and electrolyte design. Ferrocene derivatives are effective mediators for enhancing electron transfer in electrochemical biosensors.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Superoxide, hydrogen peroxide, and the gelling of phloem sap from Cucurbita pepo.

Planta·2013
Same author

Broadband optical phase shifter.

Applied optics·2010
Same author

Shearing interferometer as an interferometric filter for the reduction of scattered light.

Applied optics·2010
Same author

Room temperature vulcanizing silicone rubber as an optical element.

Applied optics·2010
Same author

Corner cube interferometer using extended sources.

Applied optics·2010
Same author

SCLERA: an Astrometric Telescope for Experimental Relativity.

Applied optics·2010

Area of Science:

  • Electrochemistry
  • Biochemistry
  • Biosensor Technology

Background:

  • Direct electrochemistry of redox proteins has been established using various electrodes.
  • Achieving rapid and reversible protein-electrode interactions requires precise electrode surface and electrolyte condition optimization.
  • The electrochemistry of complex systems like redox enzymes is an active area of research.

Purpose of the Study:

  • To explore the exploitation of redox protein electrochemistry by coupling electrode reactions with cofactor-dependent enzymes.
  • To investigate the use of mediators for enhancing slow direct electron transfer rates.
  • To highlight the role of ferrocene derivatives in advanced electrochemical biosensors.

Main Methods:

  • Utilizing modified gold, pyrolytic graphite, and metal oxide electrodes for direct protein electrochemistry.

Related Experiment Videos

  • Investigating the coupling of electrode reactions with redox enzymes using redox proteins as cofactors.
  • Employing small electron carriers, specifically ferrocene and its derivatives, as mediators to facilitate electron exchange.

    • Main Results:

    • Demonstrated successful direct electrochemistry of redox proteins at diverse electrode materials.
    • Showcased the 'well-behaved' electrochemical properties of redox proteins for enzyme coupling.
    • Confirmed the efficacy of ferrocene derivatives as mediators in enhancing electron transfer rates.

    Conclusions:

    • Direct electrochemistry of redox proteins is feasible and can be optimized through careful experimental design.
    • Mediators, particularly ferrocene derivatives, are crucial for improving electron transfer in electrochemical systems.
    • New electrochemical biosensors are leveraging ferrocene derivatives for enhanced performance.