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Ethanol-Fixed, Paraffin-Embedded Tissue Imaging: Implications for Alzheimer's Disease Research
Andrea R Kelley, Madeline Colley, Savannah Dyer
1Department of Pathology, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, Ohio 44106, United States.
Journal of the American Society for Mass Spectrometry
|August 18, 2020
Summary
Ethanol-fixed, paraffin-embedded tissue offers a solution for long-term preservation in mass spectrometry imaging (MSI). This method enables the simultaneous analysis of intact proteins and lipids, crucial for disease research like Alzheimer's disease (AD).
Area of Science:
- Biomedical research
- Analytical chemistry
- Pathology
Background:
- Mass spectrometry imaging (MSI) is vital for disease research.
- Fresh-frozen tissue is optimal for MSI but has limited preservation.
- Formalin-fixed paraffin-embedded tissue offers long-term storage but hinders direct protein imaging.
Purpose of the Study:
- To evaluate ethanol-fixed, paraffin-embedded (EFPE) tissue for MSI.
- To enable simultaneous localization of intact proteins and lipids.
- To explore implications for Alzheimer's disease (AD) research.
Main Methods:
- Developed new sample preparation methods for EFPE tissues.
- Implemented xylene-free deparaffination for lipid analysis.
- Utilized an intact protein method for visualizing amyloid-beta plaques.
Main Results:
- Broadened the information extractable from MSI experiments.
- Successfully visualized intact proteins and lipids from a single tissue source.
- Demonstrated visualization of amyloid-beta plaques in human AD brain tissue.
Conclusions:
- EFPE tissue preparation streamlines MSI workflows.
- This approach allows for comprehensive, biologically relevant data extraction.
- EFPE-MSI is a promising technique for advancing disease research, particularly for AD.
