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Standardization of plasminogen assays.

¹National Institute for Biological Standards and Control, South Mimms, Herts. U.K.

|January 1, 1988

Summary

Assessing plasminogen (plgn) potency has evolved with new assays. A British Standard for glutamic acid-plasminogen now standardizes the plasminogen-streptokinase complex assay for accurate plasma measurement.

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Area of Science:

Biochemistry
Hematology
Enzymology

Background:

Plasminogen (plgn) is a multifunctional molecule in coagulation and fibrinolysis, acting as a proenzyme with unexpressed enzymatic activity.
Traditional assays for plasminogen potency involve activation to plasmin, facing challenges in optimizing the plasminogen-activator ratio.
Previous assays used substrates like casein, fibrinogen, or fibrin, with varying results influenced by plasminogen's fibrin-binding structures.

Purpose of the Study:

To describe a novel assay for plasminogen activity measurement.
To introduce a new British Standard for glutamic acid-plasminogen.
To discuss the calibration and application of this standard in plasma plasminogen measurement.

Main Methods:

Development of an assay based on the plasminogen-streptokinase (plgn-SK) complex.
This complex directly hydrolyzes a chromogenic substrate (S-2251), bypassing activation issues.
Calibration of the British Standard using the 2nd International Reference Preparation (IRP) for plasmin.

Main Results:

The plgn-SK complex assay overcomes limitations of previous activation-dependent methods.
A British Standard for glutamic acid-plasminogen has been established for assay standardization.
The study discusses the calibration of this standard against the 2nd IRP and its utility in plasma assays.

Conclusions:

The plasminogen-streptokinase complex assay offers a more reliable method for measuring plasminogen activity.
The newly established British Standard is crucial for standardizing this improved assay.
This advancement facilitates accurate measurement of plasminogen in plasma samples.

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