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Bio-PEDOT: Modulating Carboxyl Moieties in Poly(3,4-ethylenedioxythiophene) for Enzyme-Coupled Bioelectronic
Kiattisak Promsuwan1,2,3, Lingyin Meng1, Phachara Suklim1,2,4
1Biosensors and Bioelectronics Centre, Department of Physics, Chemistry and Biology, Linköping University, SE-581 83 Linköping, Sweden.
ACS Applied Materials & Interfaces
|August 19, 2020
Summary
Researchers developed a new method to modify conducting polymers (CPs) using carboxylate molecules, enhancing their performance for organic bioelectronics (OBEs). This innovation led to a highly sensitive lactate biosensor for detecting lactate in serum samples.
Area of Science:
- Materials Science
- Electrochemistry
- Biotechnology
Background:
- Conducting polymers (CPs) are crucial for organic bioelectronics (OBEs), but their integration with biomolecules needs improvement.
- Modulating functional groups on CPs offers a pathway to enhance their properties and create stable bio-interfaces.
Purpose of the Study:
- To develop a facile approach for modulating carboxylate functional groups on PEDOT interfaces.
- To systematically evaluate the impact of various carboxylate dopants on PEDOT properties and polymerization efficiency.
- To create a stable, biofunctionalized PEDOT interface for biosensing applications.
Main Methods:
- Systematic evaluation of carboxylate-containing molecules (acetate, malate, citrate, poly(acrylamide-co-acrylate)) as counterion dopants for PEDOT.
- Characterization of PEDOT:COO- interfaces, including morphology, surface carboxylate density, and electrochemical kinetics.
- Covalent coupling of lactate dehydrogenase (LDH) enzyme to the optimized PEDOT:poly-COO- interface.
- Development and testing of a lactate biosensor using the biofunctionalized PEDOT (Bio-PEDOT).
Main Results:
- Tunable PEDOT:COO- interfaces with controlled morphology (0.33 to 0.11 μm) and surface carboxylate density (0.56 to 3.6 μM cm-2).
- Improved electrochemical kinetics and cycling stability of the modulated PEDOT interfaces.
- Successful development of a lactate biosensor with high sensitivity (8.38 μA mM-1 cm-2) and reproducibility.
- Detection of lactate in spiked serum samples with high recovery (91-96%) and low RSD (2.1-3.1%).
Conclusions:
- The carboxylate modulation strategy effectively enhances PEDOT properties for OBEs.
- The developed Bio-PEDOT interface enables stable enzyme immobilization and sensitive biosensing.
- This work paves the way for all-polymer-based OBEs in sensing, bioengineering, and biofuel cells.
