A simple enzymeless approach for Paraoxon determination using imidazole-functionalized carbon nanotubes

This work describes the application of a glassy carbon electrode (GCE) modified with imidazole functionalized carbon nanotubes (CNT-H-IMZ) for Paraoxon (PX) determination in samples of commercial, fresh and 100% orange juice. Homemade multi-walled CNTs were treated according to the Hummers procedure to oxidize graphite and later chemically functionalized with imidazole groups. Modified electrodes with CNT-H-IMZ presented a high peak current of PX reduction and an electrocatalytic effect in comparison to the other electrodes. This behavior was associated with the synergistic contribution of IMZ and CNT that increases the electrochemical activity of PX. Repeatability and reproducibility studies showed that the relative peak current values did not show significant differences between them, less than 10%, and it was possible to define that the diffusional process is the mechanism that limits the electrode mass transport. After the optimization of parameters inherent to the methodology and the voltammetric technique, the proposed device presented a linear region of 1.0 to 16.0 μM^-1 (R² = 0.99), presenting LOD and LOQ as 120 and 400 nM^-1, respectively. The method proposed was successfully applied to PX determination in spiked samples.