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Updated: Dec 11, 2025

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

BASIC: A Simple and Accurate Modular DNA Assembly Method.

Marko Storch^1,2,3, Ari Dwijayanti^4,5, Haris Mallick^4,5

¹Department of Life Sciences, Imperial College London, London, UK. m.storch@imperial.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)

|August 19, 2020

Summary

This summary is machine-generated.

The Biopart Assembly Standard for Idempotent Cloning (BASIC) offers a highly accurate and robust method for DNA assembly. This versatile cloning workflow enables efficient, reproducible construction of complex genetic designs at various scales.

Keywords:

Combinatorial DNA assembly Hierarchical Idempotent Pathway engineering

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Last Updated: Dec 11, 2025

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Area of Science:

Synthetic Biology
Molecular Biology
Bioengineering

Background:

DNA assembly methods are crucial for synthetic biology workflows.
Existing methods can suffer from low accuracy, complexity, or lack of modularity.
Standardization is needed to improve reproducibility and part sharing.

Purpose of the Study:

To introduce and describe the Biopart Assembly Standard for Idempotent Cloning (BASIC) method.
To highlight the accuracy, robustness, and modularity of the BASIC system.
To present BASIC as a solution for efficient and reproducible DNA assembly.

Main Methods:

BASIC utilizes standardized DNA linkers with flanking prefix and suffix sequences (18 bp).
Bioparts are stored in a universal BASIC format, allowing seamless integration.
Functional linkers (e.g., RBS, peptide linkers) enhance modularity.
A single-tier format enables hierarchical assembly without reformatting.

Main Results:

BASIC achieves 99% accuracy for typical four-part assemblies.
The method supports assembly of up to seven parts per round.
BASIC facilitates efficient combinatorial and complex assembly designs.
The universal format enables creation of compressed, shareable biopart libraries.

Conclusions:

BASIC is a simple, robust, and highly accurate DNA assembly standard.
Its modularity and single-tier format support efficient hierarchical cloning.
BASIC promotes part sharing and enhances reproducibility in synthetic biology.
The method is suitable for both bench-scale and biofoundry applications.