SERS-fluorescence-superresolution triple-mode nanoprobe based on surface enhanced Raman scattering and surface...

Multifunctional nanoprobes play important roles in cell imaging and sensing. Here, we present a novel optical nanoprobe based on surface enhanced Raman scattering (SERS) and surface enhanced fluorescence (SEF), which can realize the SERS-fluorescence and superresolution triple-mode imaging of cancer cells. Compared with other previously reported multifunctional nanoprobes, the proposed nanoprobe holds two exquisite properties. The first one is that, in addition to normal SERS and fluorescence imaging, the nanoprobe can also be used for single molecule localization microscopy (SMLM) imaging, which helps compensate for the diffraction limited spatial resolution of normal SERS and fluorescence imaging. The second one is that, other than simple fluorescence, SEF is used in the nanoprobe to produce a stronger signal for fluorescence imaging and, more importantly, better photo-switching for SMLM imaging. In the experiment, we optimized the structure of the nanoprobe to obtain the best SEF effect. With the optimal structure, the triple-mode imaging of a breast cancer cell line (SKBR3) is realized. Since such triple-mode imaging of cancer cells has never been achieved before, we believe that the presented nanoprobe holds great potential for cancer cell targeting or the investigation of cell-nanomaterial interactions.