Related Experiment Video
Updated: Aug 5, 2026
06:00
Portable Paper-Based Immunoassay Combined with Smartphone Application for Colorimetric and Quantitative Detection of Dengue NS1 Antigen
Published on: January 26, 2024
A portable smartphone-based fluorescence biosensing strategy for exosome detection
Sijie Zhang1, Shijie Zhang1, Hongju Shi1
1Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University, Nanjing, 210096 Jiangsu, People's Republic of China.
Nanotechnology
|July 28, 2026
Summary
Researchers developed a portable smartphone-based platform for rapid exosome detection. This innovation enables sensitive and quantitative analysis of exosomes (extracellular vesicles) for improved disease diagnostics at the point-of-care.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Molecular Diagnostics
Background:
- Exosomes are vital for intercellular communication and serve as potential disease biomarkers.
- Current exosome detection methods face challenges in speed, portability, and quantitative accuracy.
- Existing techniques often require bulky equipment, limiting point-of-care applications.
Purpose of the Study:
- To develop a portable, smartphone-based fluorescence sensing platform for exosome detection.
- To overcome limitations of conventional assays regarding portability, quantitative analysis, and ease of use.
- To enable rapid, on-site biomedical diagnostics using exosome biomarkers.
Main Methods:
- Exosome capture utilizing CD63-specific aptamers.
- Molecular recognition via FAM-labeled, tumor-specific aptamers for fluorescence signaling.
- A miniaturized optical readout device for fluorescence acquisition and quantitative analysis.
Main Results:
- The platform achieved exosome detection within 40 minutes.
- A limit of detection of 7.124 × 10^6 particles/mL was demonstrated.
- Clinical serum sample analysis showed high reproducibility and accuracy.
Conclusions:
- The developed portable platform offers a rapid and simple method for exosome detection.
- It shows significant potential for on-site biomedical diagnostics and disease biomarker analysis.
- This technology addresses the need for accessible and quantitative exosome assays.
