Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Leveraging the Oryza telomere-to-telomere genome and wild-rice substitution lines for rice-quality improvement.

Current biology : CB·2026

Same author

A pilot study of Galectin-3 targeting in chronic kidney fibrosis and kidney function decline.

BMC nephrology·2026

Same author

ENPP1 blockade with a humanized monoclonal antibody enhances renal repair after acute kidney injury.

Cell stem cell·2026

Same author

Genome editing precisely boosts salt-tolerant japonica rice yield.

Journal of integrative plant biology·2026

Same author

Phosphorylation of AEG-1 suppresses NF-κB signaling in gastric cancer.

Molecular biology reports·2026

Same author

Reevaluating survival outcomes of Mohs surgery vs wide local excision in sebaceous carcinoma: Insights from SEER with propensity score matching.

Journal of the American Academy of Dermatology·2026

Same journal

A Point-of-Care System for the Quantification of Small-Molecule Drugs in Blood.

ACS sensors·2026

Same journal

A Fungal Bioluminescent Pathway (FBP)-Based Yeast Biosensor for Caffeic Acid Quantification in Food and Beverages.

ACS sensors·2026

Same journal

Additively Manufactured <i>in planta</i> Integrated Microneedle-Microfluidic Sensing: Nondestructive Electrochemical Tracking of Glucose and Water Stress in Agricultural Crop Plants.

ACS sensors·2026

Same journal

Printable Core-Shell Multifunctional Particle for Light-Enhanced Nanomolar-Level Testosterone Point-of-Care Monitoring.

ACS sensors·2026

Same journal

Robust and Sensitive Electrochemical Biosensor Based on Cascade Interface Engineering for piRNA Detection in Breast Cancer Diagnosis.

ACS sensors·2026

Same journal

CRISPR-Cas-Based Platform for Single-Step Quantification of Monoclonal Antibodies at Point-of-Care.

ACS sensors·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 9, 2025

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Real-Time Three-Dimensional Imaging of Cell-Substrate Adhesion Structures Based on Plasmonic Scattering Microscopy.

Qihao Sun¹, Zuyao Wang¹, Zongyan Zhang¹

¹School of Electronic Science and Engineering, Southest University, Nanjing 211189, China.

|August 28, 2025

Summary

This summary is machine-generated.

This study introduces a novel plasmonic scattering microscopy (PSM) technique for real-time, 3D analysis of cell-substrate interactions. The method offers label-free monitoring of cell adhesion dynamics with high spatial resolution.

Keywords:

basal cell membrane dynamics cell−substrate adhesion gap plasmonic scattering microscopy surface plasmon resonance three-dimensional reconstruction

More Related Videos

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy

Published on: January 21, 2019

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

Related Experiment Videos

Last Updated: Sep 9, 2025

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy

Published on: January 21, 2019

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

Area of Science:

Cellular Biomechanics
Biophysics
Microscopy

Background:

Real-time monitoring of cell-substrate interactions is crucial but challenging.
Understanding cell adhesion dynamics requires high-resolution imaging techniques.

Purpose of the Study:

To develop and validate a novel microscopy system for real-time, 3D analysis of the cell-substrate interface.
To investigate dynamic cell adhesion modes using label-free imaging.

Main Methods:

Integration of plasmonic scattering microscopy (PSM) with 3D image reconstruction.
Validation using microspheres with known geometries.
3D mapping of MCF-7 cell-Ag substrate interface.

Main Results:

Achieved submicrometer spatial resolution in real-time analysis.
Reconstructed images showed a mean coefficient of determination of 86%.
Identified three distinct cell-substrate contact modes and their dynamic adhesion gap ranges.

Conclusions:

The developed PSM system enables label-free, real-time monitoring of 3D cell-substrate adhesion dynamics.
The technique provides valuable insights into cell adhesion mechanisms.
Demonstrated potential as a tool for analyzing cell adhesion dynamics.