Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Integrating condensates into protein lifespan - from birth to death.

The Journal of biological chemistry·2026

Same author

The evolving role of structural biology in pharma: integration of X-ray crystallography, cryo-electron microscopy and beyond.

Acta crystallographica. Section D, Structural biology·2026

Same author

A 3D In Vitro Model of the Human Hepatobiliary Junction.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Survey of the human proteostasis network: the ubiquitin-proteasome system.

bioRxiv : the preprint server for biology·2026

Same author

Cryogenic electron tomography reveals herpesvirus capsid assembly intermediates inside the cell nucleus.

Nature communications·2026

Same author

NAC controls nascent chain fate through tunnel sensing and chaperone action.

Nature·2025

Same journal

Structure of Perinereis linea erythrocruorin reveals a compact extracellular globin megacomplex.

Structure (London, England : 1993)·2026

Same journal

Meet the author: Stephen Brohawn.

Structure (London, England : 1993)·2026

Same journal

Tetraspanins bring Norrin into focus: Structural insights into ligand-specific Wnt signaling.

Structure (London, England : 1993)·2026

Same journal

Uncovering subtype-selective activation of the K<sub>Ca</sub>3.1 channel by SKA-111.

Structure (London, England : 1993)·2026

Same journal

Identification and structure determination of a type III-Bv CRISPR complex that post-translationally modifies an associated toxin.

Structure (London, England : 1993)·2026

Same journal

Cryo-EM structure of the Arabidopsisthaliana ribosome in translating and non-translating states.

Structure (London, England : 1993)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Dec 11, 2025

Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography

Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography

Published on: August 5, 2021

Multi-scale 3D Cryo-Correlative Microscopy for Vitrified Cells.

Gong-Her Wu¹, Patrick G Mitchell², Jesus G Galaz-Montoya¹

¹Department of Bioengineering, James H. Clark Center, Stanford University, Stanford, CA 94305, USA.

Structure (London, England : 1993)

|August 20, 2020

Summary

This summary is machine-generated.

This study presents a new 3D imaging pipeline for visualizing vitrified cells at cryogenic temperatures. The method reveals subcellular structures and organelle distribution in yeast cells without chemical fixation.

Keywords:

Airyscan microscopy Hsp104 chaperone Saccharomyces cerevisiae (yeast)amyloid cryo mill and view (cryoMAV).cryo-correlative light and electron microscopy (cryoCLEM)cryo-electron tomography (cryoET)protein aggregation protein misfolding volume cryo-focused ion bean scanning electron microscopy (cryoFIB-SEM)

More Related Videos

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

Sample Preparation by 3D-Correlative Focused Ion Beam Milling for High-Resolution Cryo-Electron Tomography

Sample Preparation by 3D-Correlative Focused Ion Beam Milling for High-Resolution Cryo-Electron Tomography

Published on: October 25, 2021

Related Experiment Videos

Last Updated: Dec 11, 2025

Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography

Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography

Published on: August 5, 2021

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

Sample Preparation by 3D-Correlative Focused Ion Beam Milling for High-Resolution Cryo-Electron Tomography

Sample Preparation by 3D-Correlative Focused Ion Beam Milling for High-Resolution Cryo-Electron Tomography

Published on: October 25, 2021

Area of Science:

Cell Biology
Microscopy
Biophysics

Background:

Traditional cell imaging requires chemical fixation and staining, which can introduce artifacts.
Three-dimensional (3D) visualization of vitrified cells offers a path to preserving native cellular structures.
Subcellular complex visualization is crucial for understanding cellular function.

Purpose of the Study:

To develop and validate an integrated imaging pipeline for multi-scale 3D visualization of vitrified cells.
To demonstrate the pipeline's capability in revealing subcellular structures and organelle distribution.
To assess the potential for large-scale phenotypic studies of frozen-hydrated specimens.

Main Methods:

Integration of three imaging modalities: cryo-fluorescence confocal microscopy, volume cryo-focused ion beam scanning electron microscopy, and transmission cryo-electron tomography.
Cryogenic temperature imaging of the same specimen.
Proof-of-concept benchmark using heat-shocked yeast cells.

Main Results:

Successful 3D visualization of organelles and subcellular structures in whole yeast cells.
Detailed ultrastructure of protein inclusions and their recruitment of fluorescently-labeled chaperone Hsp104.
Demonstration of integrated imaging across different scales.

Conclusions:

The developed pipeline enables artifact-free 3D visualization of vitrified cells at multiple scales.
This workflow can be applied to various cell types for studying healthy and diseased conditions.
The method facilitates large-scale phenotypic studies of frozen-hydrated specimens.