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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Dec 11, 2025

Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper

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Published on: April 9, 2017

SMAP: a modular super-resolution microscopy analysis platform for SMLM data

¹Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany. Jonas.ries@embl.de.

|August 21, 2020

Summary

No abstract available in PubMed .

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