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Updated: Dec 11, 2025

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
Published on: May 6, 2010
An Algorithm for the Analysis of the 3D Spatial Organization of the Genome
Francesco Gregoretti1, Alice Cortesi2, Gennaro Oliva3
1Institute for High Performance Computing and Networking, ICAR-CNR, Naples, Italy. francesco.gregoretti@icar.cnr.it.
Abstract:
We present an algorithm, and its MATLAB implementation, based on mathematical methods to detect and localize 3D multicolor DNA FISH spots in fluorescence cell image z-stacks. This algorithm provides a method to measure the relative positioning of spots in the nucleus and inter-spot distances with the aim to enrich our understanding of the 3D spatial organization of the genome within the cell nucleus.
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