Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.4K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.4K
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

1.5K
The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
1.5K
Imaging Studies IV: Magnetic Resonance Imaging01:27

Imaging Studies IV: Magnetic Resonance Imaging

166
Introduction:Magnetic Resonance Imaging, or MRI, can include a specialized imaging technique of the urinary system known as Magnetic Resonance Urography (MRU). This radiation-free technique uses strong magnetic fields and radio waves to produce detailed images with the help of a computer. MRU is particularly effective for visualizing fluid-filled structures like the kidneys, ureters, and bladder.Applications of MRI in the Genitourinary SystemKidneys and Ureters: MRI detects tumors, cysts,...
166
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

846
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
846
Magnetic Resonance Imaging01:24

Magnetic Resonance Imaging

8.8K
Magnetic resonance imaging (MRI) is a noninvasive medical imaging technique based on a phenomenon of nuclear physics discovered in the 1930s, in which matter exposed to magnetic fields and radio waves was found to emit radio signals. In 1970, a physician and researcher named Raymond Damadian noticed that malignant (cancerous) tissue gave off different signals than normal body tissue. He applied for a patent for the first MRI scanning device in clinical use by the early 1980s. The early MRI...
8.8K
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

503
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
503

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The GNMT N-terminus Couples Folate Feedback to Methyl-donor Homeostasis.

bioRxiv : the preprint server for biology·2026
Same author

[3-<sup>11</sup>C]Pyruvate PET detects alterations in cardiac pyruvate metabolism induced by doxorubicin chemotherapy.

Npj imaging·2026
Same author

Coupled-enzyme assay for MTAP activity in biological samples.

Analytical biochemistry·2026
Same author

Optically Detected Magnetic Resonance Based Intracellular Thermometry Using Nanodiamonds Implanted in Adherent Cancer Cells.

ACS applied nano materials·2026
Same author

Hyperpolarized [U-<sup>2</sup>H, 2-<sup>13</sup>C]Fructose Distinguishes Direct Hepatic Gluconeogenesis Through Fructose-1-Phosphate Production in Fed and Fasted States.

ACS chemical biology·2026
Same author

Polystyrene microplastic-induced pathophysiology is driven by disruption of efferocytosis.

Immunity·2026

Related Experiment Video

Updated: Dec 11, 2025

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

10.9K

Dynamic volumetric hyperpolarized 13 C imaging with multi-echo EPI.

Kofi Deh1,2, Kristin L Granlund1,2, Roozbeh Eskandari1,2

  • 1Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York, USA.

Magnetic Resonance in Medicine
|August 22, 2020
PubMed
Summary

This study presents a robust method using iterative least squares (IDEAL) reconstruction for creating dynamic, volumetric maps of hyperpolarized 13C-pyruvate and its metabolites in vivo. This technique enhances the assessment of metabolic processes across multiple organs.

Keywords:
IDEALhyperpolarized 13Cmetabolic imagingmultiecho echo-planar imaging

More Related Videos

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents
08:59

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents

Published on: April 15, 2016

7.1K
Author Spotlight: Standardization and Best Practices for Advancing Lung Imaging Using 129Xe MRI
09:08

Author Spotlight: Standardization and Best Practices for Advancing Lung Imaging Using 129Xe MRI

Published on: November 21, 2023

1.2K

Related Experiment Videos

Last Updated: Dec 11, 2025

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

10.9K
Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents
08:59

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents

Published on: April 15, 2016

7.1K
Author Spotlight: Standardization and Best Practices for Advancing Lung Imaging Using 129Xe MRI
09:08

Author Spotlight: Standardization and Best Practices for Advancing Lung Imaging Using 129Xe MRI

Published on: November 21, 2023

1.2K

Area of Science:

  • Medical Imaging
  • Metabolic Imaging
  • Hyperpolarized MRI

Background:

  • Dynamic metabolic imaging provides insights into cellular function.
  • Current methods for mapping hyperpolarized tracers have limitations in resolution and speed.

Purpose of the Study:

  • To develop and validate a method for generating dynamic, volumetric maps of hyperpolarized [1-13C]pyruvate and its metabolic products in vivo.
  • To assess the efficacy of iterative least squares (IDEAL) reconstruction for this purpose.

Main Methods:

  • Iterative least squares (IDEAL) reconstruction was applied to multiecho echo-planar imaging (EPI) data.
  • Phantoms and mice injected with hyperpolarized [1-13C]pyruvate were imaged on a preclinical 3T scanner.
  • Image quality was evaluated by assessing the separation of chemical species and generating time course and area-under-the-curve plots.

Main Results:

  • Increased EPI shots (one to four) improved IDEAL decomposition and chemical species separation.
  • Dynamic 3D metabolite maps of pyruvate and its products (pyruvate hydrate, lactate, bicarbonate, alanine) were successfully generated in live mice.
  • Time course and area-under-the-curve graphs for the heart, kidneys, and liver showed good agreement with existing literature.

Conclusions:

  • IDEAL decomposition of multishot multiecho 13C EPI is a robust method for high-quality dynamic volumetric mapping of hyperpolarized [1-13C]pyruvate and its metabolites.
  • This technique has significant potential for assessing multiorgan metabolic phenomena in vivo.