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Updated: Mar 6, 2026
Near-Infrared Temperature Measurement Technique for Water Surrounding an Induction-heated Small Magnetic Sphere
Published on: April 30, 2018
Optically Detected Magnetic Resonance Based Intracellular Thermometry Using Nanodiamonds Implanted in Adherent Cancer
John C Consiglio1, Rostislav Boltyanskiy1, Yuliya L Mindarava2
1Center for Molecular Imaging and Bioengineering, Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States.
Abstract:
Intracellular temperature influences cell metabolism and the tumor microenvironment, but there is a lack of consensus on basic thermal properties of cells and a shortage of reliable measurement tools. We utilized nanodiamonds (NDs) containing fluorescent Nitrogen Vacancy centers (NVs) implanted in glioblastoma cells to measure intracellular temperature via Optically Detected Magnetic Resonance (ODMR) and used dual fluorescence and bright-field imaging to establish ND-mitochondria distances. Careful ND selection, improved curve fitting, and time averaging enable repeatable measurement of cellular responses. Upon mitochondrial uncoupling with 5 μM FCCP, we reproducibly observed ODMR shifts consistent with an ∼1.7 °C temperature increase.
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