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Bioimaging using bipolar electrochemical microscopy with improved spatial resolution
Tomoki Iwama1, Kumi Y Inoue, Hiroya Abe
1Graduate School of Environmental Studies, Tohoku University, Sendai, Miyagi, Japan. kumi.inoue.b3@tohoku.ac.jp hitoshi.shiku.c3@tohoku.ac.jp.
The Analyst
|August 22, 2020
Summary
We developed bipolar electrochemical microscopy (BEM) with a closed bipolar electrode (cBPE) array for high-resolution imaging. This novel technique successfully visualized [Fe(CN)6]3- diffusion and cell respiration with excellent spatio-temporal detail.
Area of Science:
- Electrochemistry
- Microscopy
- Biosensing
Background:
- Conventional microelectrode arrays face limitations in achieving high spatio-temporal resolution.
- Direct detector connection in bipolar electrodes can hinder imaging capabilities.
Purpose of the Study:
- To develop a novel bipolar electrochemical microscopy (BEM) technique.
- To achieve high spatio-temporal resolution imaging using a closed bipolar electrode (cBPE) array and electrochemiluminescence (ECL).
Main Methods:
- Fabrication of a cBPE array using gold deposition in a track-etched membrane via electroless plating.
- Arrangement of individual cBPEs at 41 μm intervals for high electrode density.
- Utilizing an electrochemiluminescence (ECL) detection system with the BEM setup.
Main Results:
- Successful fabrication of a high-density cBPE array.
- Demonstration of high spatio-temporal resolution imaging of [Fe(CN)6]3- diffusion.
- Effective imaging of MCF-7 spheroid respiratory activity using the developed BEM system.
Conclusions:
- The developed BEM technique with a cBPE array offers superior spatio-temporal resolution compared to conventional methods.
- This approach enables detailed visualization of electrochemical processes and biological activities at the microscale.
- BEM holds significant potential for advanced electrochemical and biological imaging applications.
