MS Analysis of DNA Modifications in Urinary/Body Fluids
Aleksandra Skalska1, Agnieszka Siomek-Gorecka1, Ryszard Olinski1
1Department of Clinical Biochemistry, Faculty of Pharmacy, Nicolaus Copernicus University in Toruń, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland.
Methods in Molecular Biology (Clifton, N.J.)
|August 22, 2020
Summary
Mass spectrometry methods analyze DNA modifications in urine. Two new chromatographic techniques simultaneously determine modified DNA bases and nucleosides in a single sample.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Molecular Biology
Background:
- Mass spectrometry is a powerful tool for analyzing DNA modifications in biological samples.
- Accurate quantification of modified DNA bases and nucleosides in body fluids is crucial for understanding various biological processes and disease states.
Purpose of the Study:
- To describe two novel chromatographic methods for the simultaneous determination of modified DNA bases and nucleosides in human urine.
- To enable comprehensive analysis of DNA modifications within a single urine sample.
Main Methods:
- Development and application of isotope-dilution automated online two-dimensional ultraperformance liquid chromatography with tandem mass spectrometry (2D-UPLC-MS/MS).
- Utilizing high-performance liquid chromatography coupled with gas chromatography and mass spectrometry (HPLC/GC/MS) for comprehensive analysis.
Main Results:
- Successful simultaneous determination of multiple modified DNA bases and nucleosides in urine samples.
- Demonstration of the efficacy of both 2D-UPLC-MS/MS and HPLC/GC/MS for this purpose.
Conclusions:
- The described methods provide efficient and simultaneous analysis of DNA modifications in urine.
- These techniques advance the capability to study DNA damage and repair markers in clinical and research settings.
Related Concept Videos
01:21Mass Spectrometry: Complex Analysis
1.4K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.4K
01:19MALDI-TOF Mass Spectrometry
6.2K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
6.2K