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Updated: Dec 11, 2025

Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
Opening up the Toolbox: Synthesis and Mechanisms of Phosphoramidates
Emeka J Itumoh1,2,3, Shailja Data1,3, Erin M Leitao1,3
1School of Chemical Sciences, The University of Auckland, 23 Symonds Street, Auckland 1010, New Zealand.
Abstract:
This review covers the main synthetic routes to and the corresponding mechanisms of phosphoramidate formation. The synthetic routes can be separated into six categories: salt elimination, oxidative cross-coupling, azide, reduction, hydrophosphinylation, and phosphoramidate-aldehyde-dienophile (PAD). Examples of some important compounds synthesized through these routes are provided. As an important class of organophosphorus compounds, the applications of phosphoramidate compounds, are also briefly introduced.
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