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Related Experiment Video

Updated: Dec 11, 2025

Brain Pericyte Calcium and Hemodynamic Imaging in Transgenic Mice In Vivo

Brain Pericyte Calcium and Hemodynamic Imaging in Transgenic Mice In Vivo

Published on: November 20, 2021

Piezo1-Mediated Ca²⁺ Activities Regulate Brain Vascular Pathfinding during Development.

Ting-Ting Liu¹, Xu-Fei Du², Bai-Bing Zhang²

¹Institute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Shanghai Research Center for Brain Science and Brain-Inspired Intelligence, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China; University of Chinese Academy of Sciences, 19A Yu-Quan Road, Beijing 100049, China.

|August 23, 2020

Summary

This summary is machine-generated.

Endothelial tip cells guide blood vessel growth. Piezo1 channels control calcium signals in these cells, regulating branch dynamics and enabling proper brain vascular development in zebrafish.

Keywords:

Ca(2+) activity Piezo1 brain vessel calpain endothelial tip cell mechanical force nitric oxide synthase pathfinding vascular development zebrafish

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Area of Science:

Vascular biology
Cellular mechanotransduction
Developmental biology

Background:

Endothelial tip cells (ETCs) are crucial for guiding vascular sprouts during angiogenesis.
The precise mechanisms governing ETC pathfinding, essential for brain vascular patterning, are not fully understood.

Purpose of the Study:

To investigate the role of Piezo1-mediated calcium (Ca2+) signaling in endothelial tip cell pathfinding during zebrafish brain vascular development.

Main Methods:

Utilized zebrafish models to observe brain vascular development.
Investigated calcium transients in ETCs using live imaging.
Examined the effects of Piezo1 mutations and downstream signaling pathways (calpain, nitric oxide synthase) on ETC behavior and vascular patterning.

Main Results:

ETCs exhibit spontaneous local Ca2+ transients at primary branches.
High-frequency Ca2+ transients promote branch retraction via calpain.
Low-frequency Ca2+ transients promote branch extension via nitric oxide synthase.
Ca2+ transients are primarily mediated by the mechanosensitive Piezo1 channel.
Piezo1 dysfunction significantly impairs ETC pathfinding and brain vascular patterning.

Conclusions:

Piezo1-mediated Ca2+ signaling is a key regulator of endothelial tip cell branch dynamics.
This signaling pathway is essential for successful pathfinding and brain vascular development.
Highlights a novel role for Piezo1 and Ca2+ in the intricate process of vascular patterning.