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Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

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Published on: February 1, 2022

Electronic biosensors based on EGOFETs.

Mohammad Y Mulla¹, Luisa Torsi², Kyriaki Manoli³

¹Research Institutes of Sweden (RISE), Norrköping, Sweden.

Methods in Enzymology

|August 24, 2020

Summary

This summary is machine-generated.

Electrolyte gated organic field effect transistors (EGOFETs) offer ultrasensitive, label-free detection for point-of-care diagnostics. This research details EGOFETs for tailored bioelectronic sensors, including an "electronic tongue" for enantiomer differentiation.

Keywords:

Biofunctional layer Biological olfactory systems Biosensors Electrolyte-gated organic field effect transistors Electronic sensing Enantiomeric differentiation Odorant binding proteins Organic bioelectronics Self assembled monolayer

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Area of Science:

Bioelectronic Sensors
Organic Electronics
Transducer Technology

Background:

Growing demand for cost-effective, sensitive, and rapid point-of-care bioelectronic sensors.
Electrolyte gated organic field effect transistors (EGOFETs) are promising transducers due to their label-free, direct electronic transduction capabilities.
EGOFETs offer miniaturization and fast data processing, with inherent signal amplification properties.

Purpose of the Study:

To discuss the main aspects of EGOFET transducers.
To provide examples of tailoring EGOFET interfaces for specific functionalities.
To present the development of an electronic tongue for enantiomer differentiation using EGOFETs and odorant binding proteins (OBPs).

Main Methods:

Utilizing EGOFETs as the core sensing platform.
Immobilizing specific bioreceptors onto the transistor interface to ensure selectivity.
Coupling EGOFET devices with odorant binding proteins (OBPs) for enantiomer recognition.

Main Results:

Demonstrated the ability of EGOFETs to transduce bio-recognition events electronically and label-free.
Showcased methods for customizing EGOFET interfaces for desired sensing applications.
Successfully developed an EGOFET-based electronic tongue capable of differentiating enantiomers.

Conclusions:

EGOFETs are versatile and effective transducers for advanced bioelectronic sensors.
Tailoring device interfaces is crucial for achieving specific sensing functionalities.
The developed electronic tongue represents a significant advancement in enantiomer analysis.