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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Reporter Genes02:11

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Related Experiment Video

Updated: Dec 11, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

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Imaging mRNA trafficking in living cells using fluorogenic proteins.

Jiahui Wu1, Samie R Jaffrey1

  • 1Department of Pharmacology, Weill Cornell Medicine, Cornell University, New York, NY, 10065, USA.

Current Opinion in Chemical Biology
|August 24, 2020
PubMed
Summary

Imaging messenger RNAs (mRNAs) is vital for understanding cellular functions. New methods using tethered fluorescent proteins and fluorogenic aptamers, including novel RNA-regulated fluorescent proteins, simplify mRNA localization and dynamics studies.

Keywords:
AptamersFluorescent proteinsFluorogenic proteinsImagingRNA

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Messenger RNAs (mRNAs) regulate diverse cellular functions through precise intracellular localization.
  • Spatiotemporal control of protein expression relies on mRNA localization, making it critical for cell function.
  • Visualizing mRNA localization and dynamics is essential for understanding cellular processes.

Purpose of the Study:

  • To review current methods for imaging mRNA localization and dynamics.
  • To highlight the utility of tethered fluorescent proteins and fluorogenic aptamers for mRNA imaging.
  • To introduce and discuss the advantages of novel RNA-regulated fluorescent proteins ('fluorogenic proteins') in simplifying mRNA imaging.

Main Methods:

  • Utilizing tethered fluorescent proteins for mRNA visualization.
  • Employing fluorogenic aptamers for specific mRNA tagging and detection.
  • Leveraging newly developed RNA-regulated fluorescent proteins for enhanced mRNA imaging.

Main Results:

  • Tethered fluorescent proteins and fluorogenic aptamers provide effective means for mRNA imaging.
  • RNA-regulated fluorescent proteins offer a simplified approach to mRNA imaging experiments.
  • These techniques enable detailed studies of mRNA localization and dynamics within living cells.

Conclusions:

  • Advanced imaging techniques are crucial for dissecting the role of mRNA localization in cellular function.
  • Fluorogenic proteins represent a significant advancement, simplifying complex mRNA imaging protocols.
  • Improved methods for mRNA visualization will accelerate discoveries in cell biology and molecular medicine.