Related Experiment Video

Updated: Dec 11, 2025

Engineering Antiviral Agents via Surface Plasmon Resonance
13:00

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

2.6K

Mutations in the phosphorylation sites of SARS-CoV-2 encoded nucleocapsid protein and structure model of

H Y Lim Tung1, Pierre Limtung1

  • 1Peptide and Protein Chemistry Research Laboratory, Nacbraht Biomedical Research Institute, 3164 21st Street Suite 122, Astoria (NYC), NY, 11106, USA.

Biochemical and Biophysical Research Communications
|August 25, 2020
PubMed

Insights

Mutations in the SARS-CoV-2 nucleocapsid protein

Area of Science:

  • Molecular Biology
  • Virology
  • Biochemistry

Background:

  • Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) causes COVID-19.
  • Effective prevention strategies beyond population movement restrictions are lacking.
  • Understanding SARS-CoV-2 molecular biology is crucial for control.

Purpose of the Study:

  • To investigate mutations in SARS-CoV-2 nucleocapsid protein phosphorylation sites.
  • To present a structure-based model of Protein 14-3-3 interaction with the nucleocapsid protein.
  • To elucidate the role of these interactions in viral genome regulation.

Main Methods:

  • Analysis of SARS-CoV-2 nucleocapsid protein mutations in phosphorylation sites.
  • Structure-based modeling to predict protein-protein interactions.
  • Biochemical analysis of protein function and cellular response.

Main Results:

  • Mutations were identified within a critical phosphorylation-dependent interaction site on the nucleocapsid protein.
  • A model demonstrates how Protein 14-3-3 sequesters and inhibits nucleocapsid protein function.
  • These interactions are linked to the control of viral genome replication, transcription, and packaging.

Conclusions:

  • Phosphorylation of SARS-CoV-2 nucleocapsid protein and its interaction with Protein 14-3-3 represent a host cell defense mechanism.
  • Targeting these interactions could offer novel therapeutic strategies against SARS-CoV-2.
  • Further research into viral protein phosphorylation and host interactions is warranted.
Keywords:
C-TAK1Nucleocapsid proteinPhosphorylation dependent binding and sequestrationProtein 14-3-3Protein kinasesSARS-CoV-2

More Related Videos

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

19.2K
Exploring Caspase Mutations and Post-Translational Modification by Molecular Modeling Approaches05:56

Exploring Caspase Mutations and Post-Translational Modification by Molecular Modeling Approaches

Published on: October 13, 2022

1.6K

Related Concept Videos

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
8.4K
Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
14.5K
Phosphorylation01:02

Phosphorylation

The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
53.3K
Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.5K
Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
17.7K
Conjugated Proteins02:50

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
25.1K