Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

10.9K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
10.9K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

14.4K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
14.4K
IR Spectrometers01:25

IR Spectrometers

2.0K
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

NV716 acts as an envelope-active adjuvant that enhances antibiotic accumulation in Pseudomonas aeruginosa.

npj antimicrobials and resistance·2026
Same author

CLN5 disease-causing mutations impact lysosomal biology by affecting intracellular degradation and protein trafficking.

Biochimica et biophysica acta. Molecular basis of disease·2026
Same author

Natural phenolic autofluorescence reveals cell wall-specific distribution of hydroxycinnamates in Brachypodium distachyon.

Journal of experimental botany·2026
Same author

Interpreting medieval paints through lac dye reconstructions using synchrotron UV-VIS luminescence spectral imaging and synchrotron infrared spectroscopy.

Talanta·2026
Same author

Determining Lysosomal Enzyme Activity Using Fluorogenic Probes.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

BeStSel: analysis site for protein CD spectra-2025 update.

Nucleic acids research·2025

Related Experiment Video

Updated: Dec 11, 2025

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry
08:51

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

Published on: September 15, 2020

4.4K

FTIR micro-spectroscopy using synchrotron-based and thermal source-based radiation for probing live bacteria.

Julie Meneghel1, Stéphanie Passot1, Frédéric Jamme2

  • 1INRAE, AgroParisTech, UMR SayFood, Université Paris-Saclay, 78850, Thiverval-Grignon, France.

Analytical and Bioanalytical Chemistry
|August 26, 2020
PubMed
Summary

Fourier transform infrared (FTIR) spectroscopy now analyzes single live bacteria in water. New methods overcome water

Keywords:
Aqueous environmentFTIRFreezingLactic acid bacteriaPopulation heterogeneity

More Related Videos

Soft Lithographic Procedure for Producing Plastic Microfluidic Devices with View-ports Transparent to Visible and Infrared Light
10:26

Soft Lithographic Procedure for Producing Plastic Microfluidic Devices with View-ports Transparent to Visible and Infrared Light

Published on: August 17, 2017

13.7K
A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

24.3K

Related Experiment Videos

Last Updated: Dec 11, 2025

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry
08:51

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

Published on: September 15, 2020

4.4K
Soft Lithographic Procedure for Producing Plastic Microfluidic Devices with View-ports Transparent to Visible and Infrared Light
10:26

Soft Lithographic Procedure for Producing Plastic Microfluidic Devices with View-ports Transparent to Visible and Infrared Light

Published on: August 17, 2017

13.7K
A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

24.3K

Area of Science:

  • Biophysics
  • Spectroscopy
  • Microbiology

Background:

  • Fourier transform infrared (FTIR) spectroscopy offers non-invasive cell analysis without dyes.
  • Studying single live bacteria in water is challenging due to water's infrared absorption and bacterial size.

Purpose of the Study:

  • To develop and apply novel FTIR setups for analyzing live bacteria in aqueous environments at various spatial resolutions.
  • To investigate differences between bacterial strains and within populations.

Main Methods:

  • Developed two FTIR setups: one using attenuated total reflection microscopy with synchrotron radiation for single-cell analysis (1 μm² spot size).
  • The second setup employed transmission microscopy with a liquid micro-chamber for analyzing cell clusters.
  • Applied both methods to two lactic acid bacterium strains with differing cryo-resistances.

Main Results:

  • Both FTIR methods successfully discriminated between the two bacterial strains.
  • Revealed significant population heterogeneity among bacteria at different spatial resolutions.
  • Multivariate analysis showed cryo-sensitive cells had higher heterogeneity and α-helix protein content.
  • Identified phosphate and peptidoglycan bands in cell envelopes as potential markers for environmental resistance.

Conclusions:

  • Novel FTIR approaches enable detailed analysis of live bacteria in aqueous environments.
  • FTIR spectroscopy can reveal strain-specific characteristics and population heterogeneity.
  • Cell envelope components may serve as indicators of bacterial stress resistance.