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Determining Lysosomal Enzyme Activity Using Fluorogenic Probes
Etienne Sauvageau1, Stephane Lefrancois2,3,4
1Centre Armand-Frappier Santé Biotechnologie, Institute national de la recherche scientifique, Laval, QC, Canada.
Methods in Molecular Biology (Clifton, N.J.)
|October 13, 2025
Summary
This chapter details methods for measuring lysosomal enzyme activity in cells or cell lysates. These assays are crucial for understanding cell biology and developing new therapeutics for lysosomal diseases.
Area of Science:
- Cell Biology
- Biochemistry
- Molecular Biology
Background:
- Lysosomes are vital organelles for cellular degradation.
- Lysosomal lumen enzymes are essential for breaking down biological molecules.
- Dysfunctional lysosomes are implicated in various diseases.
Purpose of the Study:
- To provide detailed methodologies for quantifying lysosomal enzyme activity.
- To enable the study of fundamental cellular processes.
- To assess the efficacy of therapeutic interventions targeting lysosomal function.
Main Methods:
- Measurement of lysosomal enzyme activity in cell lysates.
- Assay development for intact cell-based lysosomal enzyme activity detection.
Main Results:
- Established protocols for lysosomal enzyme activity assessment.
- Demonstrated utility in studying cell biology and therapeutic effects.
Conclusions:
- Accurate measurement of lysosomal enzyme activity is feasible in various cellular contexts.
- These methods support research in cell biology and drug discovery for lysosomal disorders.
