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Computer-Aided Wet-Spinning.

Dario Puppi1, Federica Chiellini2

  • 1BIOLab Research Group, Department of Chemistry and Industrial Chemistry, University of Pisa, UdR INSTM Pisa, Pisa, Italy. dario.puppi@unipi.it.

Methods in Molecular Biology (Clifton, N.J.)
|August 26, 2020
PubMed
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Computer-aided wet-spinning (CAWS) fabricates biomedical scaffolds with controlled shapes and porous structures. This hybrid method combines additive manufacturing and wet-spinning for versatile scaffold design.

Area of Science:

  • Biomaterials Engineering
  • Additive Manufacturing
  • Textile Engineering

Background:

  • Biomedical scaffolds require precise control over both external shape and internal porous architecture.
  • Traditional fabrication methods often struggle to achieve simultaneous control over macroporous and microporous structures.
  • Computer-aided wet-spinning (CAWS) offers a novel hybrid approach to address these limitations.

Purpose of the Study:

  • To provide a detailed description of experimental methods for fabricating biomedical polymeric scaffolds using CAWS.
  • To demonstrate the fabrication of scaffolds with predefined external shape, size, and controlled internal porous structure.
  • To present a reference protocol for poly(ε-caprolactone)-based scaffolds adaptable for other polymers and porous designs.

Main Methods:

Keywords:
Biodegradable polymersComputer-aided wet-spinningPoly(ε-caprolactone)Polymer processingScaffold fabricationTissue engineering

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  • Utilizing computer-aided design (CAD) for external shape and macroporous structure definition.
  • Employing wet-spinning techniques to control the polymeric matrix's microporosity.
  • Integrating CAD with wet-spinning (CAWS) as a hybrid fabrication process.

Main Results:

  • Successful fabrication of poly(ε-caprolactone) scaffolds with controlled pore size and geometry.
  • Demonstration of CAWS's capability to produce scaffolds with specific external dimensions and internal porous features.
  • Establishment of processing parameters for adaptable scaffold fabrication.

Conclusions:

  • CAWS is an effective hybrid technique for creating biomedical scaffolds with tailored structural properties.
  • The described protocol allows for customization of scaffold porosity and material composition.
  • This method holds potential for developing advanced, patient-specific tissue engineering constructs.