EVALUATION OF THE RISK OF CERVICAL INTRAEPITHELIAL NEOPLASIA PROGRESSION BASED ON CELL PROLIFERATION INDEX,...

Area of Science:

• Gynecologic Oncology
• Cellular Pathology
• Virology

Background:

• Human papilloma virus (HPV) is the primary cause of cervical precancerous and cancerous lesions.
• Cervical intraepithelial neoplasia (CIN) progression may be influenced by cell proliferation, epithelial-mesenchymal transition (EMT), and co-infections.

Purpose of the Study:

• To analyze cell proliferation and EMT marker expression during CIN progression.
• To evaluate the impact of co-infections on these markers and disease progression.

Main Methods:

• Immunohistochemistry was used to detect markers: Ki67, cyclin D1, phosphohiston-H3, p63, E-cadherin, β-catenin, and vimentin.
• Expression levels were analyzed in CIN cases with and without co-infections.

Main Results:

• Ki67, phosphohiston-H3, and p63 expression increased with CIN progression.
• E-cadherin and β-catenin expression decreased, while vimentin increased in advanced CIN.
• Co-infections correlated with higher Ki67 and lower E-cadherin expression.

Conclusions:

• Ki67 and phosphohiston-H3 can reliably stratify CIN progression risk.
• p63, E-cadherin, β-catenin, and vimentin expression patterns indicate CIN progression risk.
• Co-infections are associated with increased proliferation and reduced E-cadherin, serving as additional progression markers.