APPLICATION OF ULTRASOUND TECHNOLOGY IN THE PROCESSING OF HISTOLOGICAL MATERIAL.

Area of Science:

• Histopathology
• Biomedical Engineering
• Materials Science

Background:

• Traditional histological slide preparation is time-consuming and uses hazardous reagents.
• Current methods pose environmental and occupational health risks.
• There is a need for efficient, cost-effective, and sustainable tissue processing techniques.

Purpose of the Study:

• To develop a novel, cost-effective, and eco-friendly histological tissue processing technology.
• To integrate an ultrasound device into the standard tissue processing protocol.
• To reduce the time and hazardous chemical usage in slide preparation.

Main Methods:

• Developed 28 experimental protocols for tissue processing.
• Utilized ultrasound treatment (UP200HT) on 168 autopsy tissue samples.
• Evaluated slide quality using hematoxylin-eosin staining and a 5-point pathologist scoring system.

Main Results:

• Ultrasound technology significantly reduced processing time and reagent consumption.
• The method proved to be more cost-effective and eliminated the need for xylene.
• Improved preservation of tissue antigenic structure was observed due to shorter reagent exposure.

Conclusions:

• The integration of ultrasound technology offers a beneficial advancement in histological processing.
• This interdisciplinary approach benefits medical staff, the environment, and patients through improved quality and reduced waiting times.
• The developed method represents a significant step towards sustainable and efficient histopathology practices.