Related Experiment Video
Updated: Oct 25, 2025
Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model
Published on: April 13, 2015
TUMOR INFILTRATING LYMPHOCYTES PECULIARITIES IN DIFFERENT HISTOPATHOLOGICAL AND MOLECULAR SUBTYPES OF GASTRIC
N Museridze1, A Tutisani1, G Chabradze1
1Tbilisi Medical Academy Named After P. Shotadze.
Abstract:
Gastric carcinoma represents one of the major causes of cancer related mortality worldwide. Recently, immunotherapeutic means gave the new promise for the treatment of gastric carcinoma, although, not all patients benefit from this type of treatment. Tumor infiltrating lymphocytes (TILs) are considered as one of the promising prognostic and predictive biomarkers in solid tumors. However, the presence of TILs is not well characterised in different types of gastric cancer. The aim of our study was to characterise TILs profile in different histopathological and molecular subtypes of gastric carcinomas. We used standard haematoxylin and eosin staining (H&E) for evaluation of TILs and immunohistochemistry to detect molecular markers, including CDH1, Ki67, p53 and Her2. The results of our study revealed that TILs status varies significantly in different histological and molecular subtypes of gastric adenocarcinoma, which might be the reason for different prognosis in these patients. Also, high TILs status is significantly related to the presence of p53 mutations in both enteric type and diffuse type gastric carcinomas, which can be further explored for immunotherapeutic options in these patients.
Insights
Tumor infiltrating lymphocytes (TILs) vary across gastric cancer subtypes. High TILs correlate with p53 mutations, offering potential for novel immunotherapeutic strategies in gastric carcinoma treatment.
Area of Science:
- Oncology
- Immunology
- Gastroenterology
Background:
- Gastric carcinoma is a leading cause of cancer mortality globally.
- Immunotherapy offers promise for gastric cancer treatment, but patient response varies.
- Tumor infiltrating lymphocytes (TILs) are key biomarkers, but their role in diverse gastric cancers needs characterization.
Purpose of the Study:
- To characterize the TILs profile across different histopathological and molecular subtypes of gastric carcinomas.
- To investigate the relationship between TILs and specific molecular markers in gastric cancer.
Main Methods:
- Standard hematoxylin and eosin (H&E) staining for TIL evaluation.
- Immunohistochemistry to detect CDH1, Ki67, p53, and Her2 expression.
- Analysis of TILs in relation to histological and molecular subtypes.
Main Results:
- TILs status significantly differs among gastric adenocarcinoma subtypes.
- High TILs are significantly associated with p53 mutations in both enteric and diffuse types.
- Variations in TILs may explain differing prognoses across gastric cancer subtypes.
Conclusions:
- TILs exhibit distinct patterns in various gastric cancer subtypes, impacting prognosis.
- The association between high TILs and p53 mutations presents a potential avenue for targeted immunotherapies.
More Related Videos
08:32Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
Published on: June 7, 2018
07:32Author Spotlight: Investigating Immune Cell Dynamics in the Tumor Microenvironment — Challenges and Innovations in Cancer Prognosis
Published on: April 12, 2024
